We have set up a new robotoc platform (AssayMap BRAVO) on which we will provide more and more automated sample preparation. The protocol for phosphopeptide enrichment is already established.
In February 2023, we have replaced the first generation QExactive Orbitrap system by a brand new timsTOF HT.
We start a new project in collaboration with NCCR RNA & Disease. The major histocompatibility complex-I (MHC–I) plays a central role in immune regulation by presenting short peptides of cell-internally degraded proteins on the cell surface to immune-competent CD8+ T-cells. Nonsense-mediated mRNA decay (NMD) is an evolutionary conserved mechanism destroying aberrant mRNA. Somatic mutations accumulate in cancer cells where NMD removes non-functional mRNA. Thus, a large fraction of potential neo-antigens is not produced and presented on the surface of cancer cells. We will investigate how NMD could potentially be manipulted to revert this situation and trigger an immune response against cancer cells.
(Last updated on April 3, 2023)